Tuesday, March 28, 2017

Hyperoxidation: White winemaking jujitsu

According to dictionary.com, jujitsu (or jiujitsu) is "a method developed in Japan of defending oneself without the use of weapons by using the strength and weight of an adversary to disable him."

Jujitsu training at an agricultural school in
Japan circa 1920 (Source: wikipedia.com)
Hyperoxidation, a white winemaking mechanism which utilizes oxidation effects during the juice phase of winemaking in order to avoid its effects later on in the bottle, is winemaking jujitsu.

I previously described the process whereby white wines are oxidized and the resulting effects. To summarize, the enzyme Tyrosinase (laccase in the case of botrytized must) catalyzes the formation of caftaric acid quinone, the result of the oxidation of the phenol caftaric acid. The quinone reacts with glutathione (a naturally occurring tripeptide found in grapes; and itself a powerful antioxidant) in the juice to form the colorless complex Grape Reduction Product (GRP). Once the glutathione is fully ustilized, GRP is no longer formed and oxidation proceeds unencumbered (This process is called enzymatic oxidation. For a fuller description of this process, as well as the non-enzymatic oxidation of wine, see here.). The results of oxidation in white wines are browning, loss of fruity aromas, and gain of aldehydic aromas.

According to Jackson (Wine Science), "In contrast to red wines, the limited antioxidant character of white wines (ed: tannins and anthocyanins provide substantive antioxidant capability in red wines) make them more susceptible to oxidative browning." Further, grape varieties differ markedly in the amount of phenolics released during crushing or extracted during maceration (an extremely important consideration given that phenolics are the main substrate for oxidation activity). The table below shows the levels of flavonoid accumulation during crushing or maceration of selected white varieties.

Table 1. Phenolics released/extracted during crushing/maceration
Variety Flavonoid Accumulation
Palomino Low
Sauvignon Blanc Low
Riesling Moderate
Semillon Moderate
Chardonnay Moderate
Muscat Gordo Extensive
Colombard Extensive
Trebbiano Extensive
Pedro Ximinez Extensive

In the case of hyperoxidation, the deliberate introduction of oxygen into the juice causes enzymatic oxidation of the phenols. The process entails adding large amounts of oxygen to the wine, allowing the juice to settle, and then racking the juice from the brown precipitate just prior to fermentation. This oxidation will cause browning of the juice but the phenols will have been polymerized and will precipitate out.  

Clarification is required to reduce the suspended solids to less than 1% by weight in order to remove the major part of the phenolic precipitate. This clarification must be completed before fermentation begins as the precipitate will redissolve in alcohol. The clarified juice will retain retain a brown color but this residual browning will be eliminated by the reducing conditions of alcoholic fermentation and absorption by yeasts (Schneider, Hyperoxidation: A Review, AJEV, 1998).

The brown pigment absorbed by the yeasts during alcohol fermentation will fall to the bottom of the tank with the lees and can be removed in a post-fermentation racking. Fining and/or filtration can be utilized for additional clarification if required.This process renders the wine less susceptible to in-bottle browning (due to the elimination of the phenols) as well as reduces bitterness in the wine.

Hyperoxidation requires that SO2 additions be withheld from the must as the oxidative enzymes are inhibited in its presence. For example, tyrosinase registers a 90% decrease in activity when 50 mg/L of SO is added to the must. SO2 also reduces caftaric acid quinone and enhances the solubility of phenolic molecules. . These effects will limit the extent and effectiveness of the hyperoxidation. The implementation of hyperoxidation can thus allow for the production of low-sulfur wines.

Hyperoxidation, then, uses the strength of oxidation in the early stages of winemaking to neuter the substrate in the early stages of winemaking and prevent it from becoming an oxidation resource in the bottle. Jujitsu.

©Wine -- Mise en abyme

2 comments:

  1. So… You are saturating the must with oxygen and not adding SO2 because it acts as an inhibitor. Then you rack the must of the brown precipitates. So far so good, but… How do you prevent fermentation prior to the precipitation?

    I am also confused since you say "browning will be mitigated by precipitation of the polymers before and during alcoholic fermentation" and then " Clarification must be completed well before fermentation begins or the precipitate will redissolve in alcohol" and "the browning will be mitigated by precipitation of the polymers before and during alcoholic fermentation"

    It seems you need to clarify prior to fermentation with simple racking, but then once fermentation begins any precipitation will dissolve into the alcohol, but then can be removed later from racking or filtering. So, is the precipitation prior to fermentation done mostly because the particulates are easier to remove prior to their being disolved in alcohol, or is it done to mitigate the normal enzymic oxidation that would tend to begin at the pre fermentation stage, focusing it instead on the phonic compounds?

    You mention that the reduction of phenols, besides have organoleptic benefits, reduce the potential for in bottle browning, but you don't really indicate if there will be SOME browning or loss of hues/brilliance due to hyperoxidation.

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    Replies
    1. Stephen
      In relation to your first question, the must is innoculated after being racked off the precipitate.

      In relation to your second paragraph, my presentation was inartful and I have reframed the two paragraphs into three to add clarity. You do need to clarify in order to reduce the solids to below 1% by weight in order to eliminate the major part of the phenolic precipitate. The only part of the process that you want to take to fermentation is the brown color which is absorbed by the yeast and falls out. If the precipitate redissolves into the medium then the beneficial effects of hyperoxidation are eliminated and in-bottle browning becomes a possibility once again.

      According to Schneider, "Wines obtained from oxidized musts show their normal bright color after fermentation."

      There is enough oxygen around. Asa matter of fact one could probably think of the process as an oxidation of the must where oxygen stability is established after all of the phenols have been eliminated.

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